Effects of Enzyme Rate by Variables of Enzyme Concentration, Substrate Concentraion, Temperature and pH
Title: Effects of Enzyme Rate by Variables of Enzyme Concentration, Substrate Concentraion, Temperature and pH
Category: Science & Technology / Biology | Words: 4185 | Pages: 17.8 (approximately 235 words/page)
Effects of Enzyme Rate by Variables of Enzyme Concentration, Substrate Concentraion, Temperature and pH
Abstract
This study was undertaken to determine the effects of different environments affecting the rate of reaction, PNPP (p-nitrophenyl phosphate) + H20 → PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase, which alters its active site, and thus changes its efficiency to catalyze the reaction. We measured …
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showed last 75 words of 4185 total…in µmoles)rate of reaction(in µmoles/min)
10.055 - 0 = .0553.34297250.0200578350.001337189
222.221 - 0 = .22112.99412950.0779647770.005197652
337.502 - .007 = .49528.92435250.1735461150.011569741
450.728 - .008 = .72042.005740.252034440.016802296
570.228 - .195 = .0332.06390350.0123834210.000825561
690.504 - .476 = .0281.7732060.0106392360.000709282
Appendix - Table 6. After obtaining the absorbance at 410nm as an effect of different temperatures and using the normal curve equation obtained in Figure 1, [p-nitrophenol] was calculated. The mass of p-nitrophenol was calculated as we found the concentration and volume of [p-nitrophenol] was .006 L. Rate of reaction is determined by dividing the mass of the product (µmoles) by the time we allowed the reaction to take place (15 minutes).
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