The Role of O-acetylserine Sulfhydrylase in Cysteine Biosynthesis in Salmonella tryphimurium: A structural and functional Analysis Introduction Pyridoxial 5’-phosphate (PLP) acts as a cofactor in many enzymes involved in diverse aspects of amino acid metabolism such as transamination, β/γ-elimination, β/γ-replacement and racemization. In all PLP-dependant enzymes the carbonyl group of the PLP coenzyme binds to an ε-amino group of a lysine residue in the active site, forming an internal aldimine. O-Acetylserine sulfahydrylase (OASS), isolated from …showed first 75 words of 2989 total…

…showed last 75 words of 2989 total…that a comprehensive functional analysis requires structural information on a molecular level. It has been known for decades that OASS was involved in L-cysteine biosynthesis, yet not until probing the significance of OASS’s structural components and observed conformational heterogeneity could we understand how the enzyme performed the complex and regulated catalytic activity. There is still more to be investigated and further pursuit of OASS’s dynamic structural and functional relationship will surely prove fruitful.